A soluble acid invertase is directed to the vacuole by a signal anchor mechanism

Title: A soluble acid invertase is directed to the vacuole by a signal anchor mechanism
Creator: Rae, Anne L.; Casu, Rosanne E.; Perroux, Jai M.; Jackson, Mark A.; Grof, Christopher P. L.
Relation: Journal of Plant Physiology Vol. 168, Issue 9, p. 983-989
Publisher Link: http://dx.doi.org/10.1016/j.jplph.2010.11.003
Publisher: Urban und Fischer Verlag
Resource Type: journal article
Date: 2011
Description: Enzyme activities in the vacuole have an important impact on the net concentration of sucrose. In sugarcane (Saccharum hybrid), immunolabelling demonstrated that a soluble acid invertase (β-fructofuranosidase; EC 3.2.1.26) is present in the vacuole of storage parenchyma cells during sucrose accumulation. Examination of sequences from sugarcane, barley and rice showed that the N-terminus of the invertase sequence contains a signal anchor and a tyrosine motif, characteristic of single-pass membrane proteins destined for lysosomal compartments. The N-terminal peptide from the barley invertase was shown to be capable of directing the green fluorescent protein to the vacuole in sugarcane cells. The results suggest that soluble acid invertase is sorted to the vacuole in a membrane-bound form.
Subject: saccharum; sucrose accumulation; sugarcane; vacuolar invertase; vacuole targeting
Identifier: http://hdl.handle.net/1959.13/1062595
Identifier: uon:17119
Identifier: ISSN:0176-1617
Language: eng
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